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Hence, we have taken for analysis not only enzymes of sul-fur transfer and methionine synthesis, but also enzymes responsible for the synthesis of methionine’s carbon back-bone from aspartic acid. GY-H28 旋链角毛藻 Chaetoceros curvisetus Cleve 1889. N stress also impacts dimethylsulphoniopropionate synthesis (Kettles et al., 2014) and redox sensitivity (Rosenwasser et al., 2014). Input custom motif sequence (max=1) Please use IUPAC code and group with "[]" (EX: NAWW[AT]AN) >IUPAC code Background: Publication of the first diatom genome, that of Thalassiosira pseudonana, established it as a model species for experimental and genomic studies of diatoms. The model diatom Thalassiosira pseudonana is believed to be a single species with a global distribution, but it has not been confirmed previously whether isolates from different environmental and geographic origins are genotypically and phenotypically identical. These two species were selected due to their size differences. Silicon plays important biological roles, but the mechanisms of cellular responses to silicon are poorly understood. QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. A total of 24 non-redundant chitinase genes were identified in the genome of T. pseudonana and designated TpChi1–24 based on their chromosomal locations. They are responsible for ~20% of global carbon fixation. In this study, we investigated the mechanism of high light protection in marine diatom Thalassiosira pseudonana using comparative proteomics in combination with biochemical analyses. Urea cycle. have been selected as representative models for the lower trophic organisms in marine and freshwater habitats, respectively. However, the molecular mechanisms underlying these acclimation responses of diaotms remain elusive. Background: Publication of the first diatom genome, that of Thalassiosira pseudonana, established it as a model species for experimental and genomic studies of diatoms. This CP12 similarly to its homologues in other species has some features of intrinsically disorder protein family: it behaves abnormally under gel electrophoresis and size exclusion chromatography, has a … In this study plant, bacterial and Overexpression of Thalassiosira pseudonana violaxanthin de-epoxidase-like 2 (VDL2) increases fucoxanthin while stoichiometrically reducing diadinoxanthin cycle pigment abundance Olga Gaidarenko , Dylan W. Mills , Maria Vernet , Mark Hildebrand It is usually a phytoplankton species but has also been recorded in the periphyton. Qin, Zhen, Xiaomin Xia, Guangming Mai, Yehui Tan, and Gang Li. They are responsible for È20 % of global carbon fixation. Here, we show that the same approach can be used to clone mitochondrial genomes of another microalga, Thalassiosira pseudonana. The term saxitoxin originates from the genus name of the butter clam from which it was first isolated. pseudonana was selected for this study because it is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans, and has a relatively small genome at 34 mega base pairs. BlastP simply compares a protein query to a protein database. Thalassiosira pseudonana is known to produce the most stable surfactant (surface-active agent) as compared to most other phytoplankton. BlastP simply compares a protein query to a protein database. BlastP simply compares a protein query to a protein database. The model marine diatom Thalassiosira pseudonana likely descended from a freshwater ancestor in the genus Cyclotella Abstract. Thalassiosira pseudonana is a species of marine centric diatoms. Thirteen putative carbonic anhydrase (CA) genes have been identified in the marine multipolar centric diatom, Thalassiosira pseudonana, and two of these CAs have been localized previously. Ensembl Protists is a browser for protist genomes. genome of the marine diatom, Thalassiosira pseudonana and its 129 Kbp plastid and 44 Kbp mitochondrial genomes. Thalassiosira pseudonana: Effects on growth, photosynthesis, and spectral sensitivity of photoinhibition Cristina Sobrino,1 Mary Love Ward, and Patrick J. Neale Smithsonian Environmental Research Center, Edgewater, Maryland 21037 Abstract We studied the effects of elevated CO 2 concentrations (0.03% vs. 0.1%) on light absorption, membrane The first (JGI) contained forward and reversed protein sequences derived from JGI gene models (T. pseudonana v3.0), whereas the second (6-RF) contained protein sequences derived from translation of the entire T. pseudonana v3.0 genome in all six reading frames. v3.0 finished chromsomes and unmapped sequence (May 2007): This In Thalassiosira pseudonana all measured cellular parameters were dependent on the photoadaptational status of the cells (Table 1). T. pseudonana was selected for this study because it is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans, and has a relatively small genome at 34 mega base pairs. 165030 TFs with at least one binding motif (4559 from direct experiments), out of a total of 392333 TFs from 321 families in 741 species "Differential Physiological Responses of Small Thalassiosira pseudonana and Large Thalassiosira punctigera to the Shifted-High Light and Nitrogen" Journal of Marine Science and Engineering 9, no. UTEX LB FD2. 9:151-154. Saxitoxin (STX) is a potent neurotoxin and the best-known paralytic shellfish toxin (PST). It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing because T. pseudonana is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans, and has a relatively small genome at 34 mega base pairs. Kingdom: Chromista Thalassiosira pseudonana more cells more cells 6. Preliminary studies indicate the likely metal catalyst is manganese, making this family of CAs unique. PLEASE NOTE: The Thalassiosira pseudonana genome sequence is composed of "finished chromosomes" (Thaps3) and "unmapped sequence" (Thaps3_bd), which were annotated separately.Please use both portals to make a complete analysis of the genome. An analysis of the expression and activity of silicon transporters (SITs) was done on synchronously growing cultures of the diatom Thalassiosira pseudonana to provide insight into the role these proteins play in cellular silicon metabolism during the cell cycle. A total of 318 transcripts were differentially regulated with a false discovery rate of <0.05, and a total of 136 proteins were differentially abundant ( p <0.05). Due to the great contribution of diatoms to global primary production 7, Thalassiosira pseudonana is one marine diatom that is widely distributed … Atteia et al, 2013 Conclusions In this work, we show that in the centric diatom Thalassiosira pseudonana the availability of photosynthetic electron acceptors decreases during anoxic acclimation and reaches a steady state (up to 48h). Diatoms are … Silicon plays important biological roles, but the mechanisms of cellular responses to silicon are poorly understood. BlastP simply compares a protein query to a protein database. We identified 24 chitinase genes and analyzed their sequence structural features, scaffold locations, phylogenetic relation-ships, stress-related cis-acting elements, subcellular loca-tions, and responses to abiotic stresses at the This To understand the ocean ecology of phytoplankton, genomic studies have been focused on the bacterial component of the plankton, the majority belonging to Prochlorococcus and Synechococcus, with very less focus on eukaryotic phytoplankton such as Thalassiosira pseudonana (diatom), … The Gene Ontology (GO) knowledgebase is the world’s largest source of information on the functions of genes. What color is the diatom usually? Taxonomy - Thalassiosira pseudonana (Marine diatom) (Cyclotella nana) (SPECIES) Cyclotella pseudonana T.P.Chang Discostella nana (Hustedt) T.-P.Chang, 2008 Homonyms Thalassiosira pseudonana Hasle & Heim. Silicon is a naturally occurring mineral. and Lange, C.R. Thalassiosira pseudonana and Synechococcus sp. In Thalassiosira pseudonana all measured cellular parameters were dependent on the photoadaptational status of the cells (Table 1). Options. Ashworth J, Turkarslan S, Harris M, Orellana MV, Baliga NS. QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. Ensembl Protists. Student pages authored independently, or for coursework, are not monitored further. Background and Objective: The microalaga Thalassiosira pseudonana is widely used in aquaculture sector. Thalassiosira pseudonana is a relatively small cell when compared to Thalassiosira weissflogii. Thalassiosira pseudonana cells acclimated to 20 000 ppm CO 2 were shifted to 50 ppm CO 2 to determine the rate of acclimation and to characterize the CCM under more carbon-limiting conditions. 2021. Ściana komórkowa (zwana skorupką) tych organizmów wysycona jest krzemionką stanowiącą ok. połowy suchej masy komórki. Status v3.0 finished chromsomes and unmapped sequence (May 2007): This Thalassiosira pseudonana genome … However, the molecular mechanisms underlying these acclimation responses of diaotms remain elusive. The marine centric diatom Thalassiosira pseudonana was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing because this species has served as a model for diatom physiology studies, the genus Thalassiosira is cosmopolitan throughout the world's oceans, and the genome is relatively small at 34 mega base pairs. To that end, we have previously demonstrated that the mitochondrial genome of microalgae Phaeodactylum tricornutum can be cloned and engineered in Saccharomyces cerevisiae and Escherichia coli. However, efficient gene targeting by homologous recombination (HR) to date is only reported for photosynthetic organisms in their haploid life-cycle phase and there are no examples of efficient nuclease-meditated HR in any photosynthetic … Thalassiosira pseudonana, (CCAP 1085/12; equivalent to CCMP1335), was grown in artificial sea water and F/2+ Si medium at 16 °C under continuous illumination at 50 … Thalassiosira pseudonana is a logical choice for CRISPR-Cas development. SOURCE is a unification tool which dynamically collects and compiles data from many scientific databases, and thereby attempts to encapsulate the genetics and molecular biology of genes from the genomes of Homo sapiens, Mus musculus, Rattus … Phycologia 28:120-135. Cultures were grown in 125-mL Erlenmeyer flasks on an orbital shaker under continuous illumination of cool-white fluorescent lamps at 150 μE m −2 s −1 at 18 °C. Therefore, we chose the model diatom Thalassiosira pseudonana in this study, by integrating whole transcriptome analysis with physiological-biochemical data, to reveal the molecular responding mechanisms of T. pseudonana to the toxicity of BDE-47. Welcome to MicrobeWiki. True or False? more cells (cc) photo by Metro Centric on Flickr Diatoms are able to acclimate to frequent and large light fluctuations in the surface ocean waters. Viability, pigments, and DMSP 3 28 Abstract 29 We measured membrane permeability, hydrolytic enzyme, and caspase-like activities using fluorescent cell stains30 to document changes caused by nutrient exhaustion in the 31 coccolithophore Emiliania huxleyi and the diatom Thalassiosira pseudonana, during batch- culture nutrient limitation. The second database also contained reversed forms of each amino acid sequence. QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. Strain Only Strain w/ 4 tubes of liquid 8 ppt F/2 media (+$10 USD) Strain Only Strain w/ 4 tubes of liquid 8 ppt F/2 media (+$10 USD) Qty. At low CO 2, cells possessed a CCM based on active uptake of CO 2 (70% contribution) and bicarbonate, while at high CO 2, cells were restricted to CO 2. Bibliographic References The harvesting of microalgae biomass from culture medium is the big challenge in terms of technology and economy. View General Maintenance Conditions. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand-base pair mitochondrial genomes. Unopened cells and large cell fragments were sedimented by a gentle centrifugation step at 300× g … QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. Results: Here, we demonstrate that CP12 is expressed in the marine diatom Thalassiosira pseudonana constitutively in dark-treated and in continuous light-treated cells as well as in all growth phases. Ściana komórkowa składa się z dwóch połówek zachodzących na siebie brzegami i tworzących swojego rodzaju puszkę (theca).Górna część (wieczko, epitheca) zachodzi na dolną (denko, hypotheca).Jest mocno urzeźbiona. A majority of these are taken from the databases of the International Nucleotide Sequence Database Collaboration (the European Nucleotide Archive at the EBI, GenBank at the NCBI, and the DNA Database of Japan); in some cases, the annotation has been taken directly from the websites of the data generators. Thalassiosira pseudonana is a marine centric diatom. Diatoms are unicellular, eukaryotic, phytoplankton that display a unique evolutionary history and provide major ecological contributions in marine environments. Metabolic pathways - Reference pathway [ Pathway menu | Organism menu | Pathway entry | Pathway menu | Organism menu | Pathway entry | Hide module list | User data mapping | 链藻 Thalassiosira pseudonana. Although genome sequences of a few diatoms are available, little is known about the … The marine centric diatom Thalassiosira pseudonana was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing because this species has served as a model for diatom physiology studies, the genus Thalassiosira is cosmopolitan throughout the world's oceans, and the genome is relatively small at 34 mega base pairs. Hasle, G.R. Cell N content, protein The target sequence of Thalassiosira pseudonana has been designated as 1pca for our convenience. model Thalassiosira species Thalassiosira pseudonana, making use of its completely sequenced genome [ 16]. Fatty acid desaturases from the microalga Thalassiosira pseudonana. Cleve. Thalassiosira was first described in 1873 by P.T. Thalassiosira pseudonana is a marine diatom, a unicellular photosynthetic … QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. PLEASE NOTE: The Thalassiosira pseudonana genome sequence is composed of "finished chromosomes" (Thaps3) and "unmapped sequence"(Thaps3_bd), which were annotated separately.Please use both portals to make a complete analysis of the genome. After electrophoresis, the 12% SDS-gels are transferred onto nitrocellulose membranes and revealed with enhanced chemiluminescence with α- T. pseudonana CP12 antibodies 1:10,000. Here, we used Thalassiosira pseudonana as a model organism to examine how phytoplankton adjust energy production and expenditure to cope with these multiple, interrelated environmental factors. It is eurythermal, experiencing good growth from 10–30°C, with an optimum around 21°C. We do not use these to store personal information about you.Continuing to use this website means you agree to our use of cookies. N stress also impacts dimethylsulphoniopropionate synthesis (Kettles et al., 2014) and redox sensitivity (Rosenwasser et al., 2014). pseudonana based on genomic data study. Molecular mass markers are shown on the right. genes in two diatom species, Thalassiosira weissflogii and Thalassiosira pseudonana under three different growth conditions of nitrogen (NO3 - only, NH4 + only, and NO 3-+ NH4 +). Three types of insoluble organic matrices were recently identified as integral components of the silica cell wall of the diatom Thalassiosira pseudonana: meshworks [ 5 ], … Gene names, gene IDs, chromosomal locations, exon numbers, amino acid sequence lengths, molecular weights (MW), and theoretical isoelectric points … BlastP simply compares a protein query to a protein database. In vivo fluorescence and chlorophyll per cell were clearly higher in cultures adapted to 70 than to 330 pm01 m-2 S-', while cell volume and the ratio between fluorescence and chlorophyll were smaller. 2001) and thus we employed 0.16% CO 2. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Diatoms are unicellular algae with plastids acquired by secondary endo-symbiosis. Here, we report a potential bioindicator of a shift in the resilience of a coastal and centric model diatom Thalassiosira pseudonana under elevated CO2. Interested readers are … Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. Thalassiosira pseudonana at 8, 17 and 25°C, and compared cell composition, and rates of growth (µ), 15N incorporation, calculated nitrate incorporation (the product of µ and cell N content), and the activ-ity of nitrate reductase (NR), a key enzyme involved in nitrate incorporation. Its full genome has been sequenced (Armbrust et al., 2004), making it especially useful in physiology studies associated with molecular mechanism. Marine diatoms are important primary producers that thrive in diverse and dynamic environments. Zinkbrist är en svårupptäckt näringsbrist som vanligen beror på för lågt zinkintag i kosten, men kan också uppkomma till följd av vissa sjukdomar. It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing. Here, we present morphological, biochemical, and molecular evidence of caspase-mediated, autocatalytic programmed cell death (PCD) in the diatom Thalassiosira pseudonana in response to iron starvation. proteins in Thalassiosira pseudonana Methionine belongs to the aspartate-family of amino acids. Thalassiosira pseudonana (CCMP1335) and C. cryptica (CCMP332) stock cultures were grown in ASW medium . The genus was then characterized with transmission electron microscopy in the 1950s and scanning electron microscopy in the 1960s, which led to an enhanced understanding of the defining morphological features of the genus and the subsequent recognition of over 100 species Current content: 11491 motifs. Ingestion of saxitoxin by humans, usually by consumption of shellfish contaminated by toxic algal blooms, is responsible for the illness known as paralytic shellfish poisoning (PSP).. It is a model centric diatom with a sequenced genome (first eukaryotic marine phytoplankton to be sequenced [ 2 ]) and well-established transformation systems [ 19, 20 ]. QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. Thalassiosira pseudonana. Using the model species Thalassiosira pseudonana , we conducted a detailed physiological and transcriptomic survey to measure the recurrent transcriptional changes that … Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. Thalassiosira pseudonana is a species of marine centric diatoms.It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing.T. (2015) Pan-transcriptomic analysis identifies coordinated and orthologous functional modules in the diatoms Thalassiosira pseudonana and … Cultures were grown in 125-mL Erlenmeyer flasks on an orbital shaker under continuous illumination of cool-white fluorescent lamps at 150 μE m −2 s −1 at 18 °C. The response of central carbon metabolism under N starvation in Thalassiosira pseudonana differs from that in green algae and higher plants, and bears closer resemblance to the cyanobacteria (Hockin et al., 2012). Thalassiosira pseudonana seems to be much more sensitive to high CO 2 than is P. tricornutum because T. pseudonana did not grow in 5% CO 2 (data not shown). We report the first analysis of cell cycle arrest and recovery from silicon starvation in the diatom Thalassiosira pseudonana using whole genome microarrays. They do so, in theory, by sensing changing conditions and adapting their physiology accordingly. Artificial coloring denotes chlorophyll (blue) and DNA (red). Thalassiosira pseudonana is a species of marine centric diatoms. It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing. Zinkbrist är vanligare i fattiga länder. The identity of Thalassiosira pseudonana The combination of a relatively nondescript morphology and a series of three name changes over the years [26-29] has led to some uncertainty about the identity of T. pseu-donana (Additional file 3). A feature of diatoms is the urea cycle, which links them evolutionarily to animals. Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. 2012-12-10 13:18:18 Ann-Turi Skjevik - Updated media metadata for Thalassiosira pseudonana_1.jpg 2012-12-10 13:17:36 Ann-Turi Skjevik - Added media: Thalassiosira pseudonana_1.jpg Nordic Microalgae is developed and operated by the Swedish Meterological and Hydrological Institute (SMHI) with funding from the Swedish LifeWatch project . Virtually every ensuing study has treated T. pseudonana as a marine diatom, with genomic and experimental data valued for their insights into the ecology and evolution of diatoms in the world's oceans. Thalassiosira pseudonana is a species of marine centric diatoms. It was chosen as the first eukaryotic marine phytoplankton for whole genome sequencing. T. pseudonana was selected for this study because it is a model for diatom physiology studies, belongs to a genus widely distributed throughout the world's oceans,... Ecology: Thalassiosira pseudonana is a cosmopolitan species typically found in freshwater and coastal brackish habitats. Thalassiosira pseudonana, (CCAP 1085/12; equivalent to CCMP1335), was grown in artificial sea water and F/2+ Si medium at 16 °C under continuous illumination at 50 μmol photon m −2 s −1, photosynthetically active radiation, as described earlier 12. En vuxen kropp innehåller normalt upp till 2 gram zink, men skattningen försvåras av zinkets höga utbredning i kroppen. Diatoms are able to acclimate to frequent and large light fluctuations in the surface ocean waters. The first, an alpha CA (TpαCA1), was localized in the chloroplast stroma; the second, a zeta-type CA (TpζCA1), was localized to the periplasmic space. View Algae Details. Thalassiosira pseudonana is a species of marine centric diatom. 20 µg crude extract from T. pseudonana cells grown under light (lane L) or dark (lane D). Silicon supplements are also used as medicine. We report the … Western blot of CP12 expression. In vivo fluorescence and chlorophyll per cell were clearly higher in cultures adapted to 70 than to 330 |xmol m^2 s_1, while cell volume and the ratio between fluorescence and chlorophyll were smaller. (1962) The morphology of Thalassiosira fluviatilis from the polluted inner Oslofjord Nytt Mag. BlastP simply compares a protein query to a protein database. Quiz more cells 1. $ 125.00. They all look alike. Bot. Rubisco and PEPC activities both decreased exponentially with a time constant of 0.086 (0.044) and 0.064 (0.035) h −1 , respectively (Fig. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Time-dependent cellular growth was measured upon exposure to both AgNP and silver nitrate (AgNO 3). Tryptophan metabolism - Reference pathway [ Pathway menu | Organism menu | Pathway entry | User data mapping] Pathway menu | Organism menu | Pathway entry | User data mapping] Synchronized growth of Thalassiosira pseudonana (Bacillariophyceae) provides novel insights into cell-wall synthesis processes in relation to the cell cycle. In the present study, cloning and … 2007; 43 : … Acidification of the ocean due to high atmospheric CO2 levels may increase the resilience of diatoms causing dramatic shifts in abiotic and biotic cycles with lasting implications on marine ecosystems. Furthermore, the environmental factors that trigger sexual reproduction in diatoms are not understood. Thalassiosira pseudonana (CCMP1335) and C. cryptica (CCMP332) stock cultures were grown in ASW medium . Abstract Thalassiosira pseudonana is a marine Bacillariophyta commonly used as live feed in mariculture. The mission of the GO Consortium is to develop a comprehensive, computational model of biological systems, ranging from the molecular to the organism level, across the multiplicity of species in the tree of life. 5 a). The genus 19, has … The reproductive strategy of diatoms includes asexual and sexual phases, but in many species, including the model centric diatom Thalassiosira pseudonana, sexual reproduction has never been observed. Thalassiosira pseudonana desaturase The algae, as a group, represent the third largest aquaculture crop (after freshwater fish and molluscs) in the world today [ [ 1 , 2 ] ]. Thalassiosira pseudonana is a centric diatom that belongs to the diverse algal group, likely arose from a common secondary endosymbiotic event, involving at least five different genomes. Many foods including water, beer, coffee, and some vegetables naturally contain silicon. Diatom skeleton's are what create diatomacious earth. a Dark and light conditions. The enzyme LCIP63 (Low CO 2-inducible protein of 63 kDa) was isolated from a marine diatom, Thalassiosira pseudonana, and shown to have robust CA activity. Thalassiosira pseudonana Tig19 cells were harvested by centrifugation and disrupted at a constant working pressure of 90 MPa using a French Press. MicrobeWiki is a free wiki resource on microbes and microbiology, authored by students at many colleges and universities.Curated pages such as those linked to the Taxonomy Index are reviewed and updated by microbiologists at Kenyon College. Instead, 0.18% CO 2 was previously shown to be not toxic but was enough to repress high-affinity photosynthesis (Burkhardt et al. Thalassiosira pseudonana is a logical choice for CRISPR-Cas development. Zhao Y, Tang X, Lv M, Liu Q, Li J, Zhang B, Li L, Zhang X, Zhao Y. Aquat Toxicol, 229:105669, 25 Oct 2020 Cited by: 0 articles | PMID: 33142158 QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. The growth rate and biochemical composition of microalgae are highly influenced by environmental factors such as, irradiance and nutrient availability. Though the exact mechanism of the highly uniform deposition of silica is as yet unknown, the Thalassiosira pseudonana genes linked to silaffins are being looked to as targets for genetic control of nanoscale silica deposition. Hasle, G.R. Publication of the first diatom genome, that of Thalassiosira pseudonana, established it as a model species... Background. Thalassiosira pseudonana Taxonomy ID: 35128 (for references in articles please use NCBI:txid35128) current name The molecular response mechanisms of a diatom Thalassiosira pseudonana to the toxicity of BDE-47 based on whole transcriptome analysis. Please cite the following if this resource has been helpful in your research. It is a model centric diatom with a sequenced genome (first eukaryotic marine phytoplankton to be sequenced [2]) and well-established transformation systems [20]. BlastP simply compares a protein query to a protein database. In this study, we investigated the mechanism of high light protection in marine diatom Thalassiosira pseudonana using comparative proteomics in combination with biochemical analyses.

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